2024 Enzyme Engineering    Mid-term Re-exam    May 14, 2024

Solve the following problems.  Clearly indicate the units in your answers.

1. Calculate the activation energy based on the following graph [20점].

2. The reaction velocity of the reaction A+B → C+D at 60oC is triple as great as the velocity at 40oC. Calculate the activation energy. The Arrhenius equation is k=k0 e-Ea/RT [20점].

3. Calculate enzyme activity as units/mg for the following reaction [20점].

Substrate: sucrose (G-F)    -Product: 1-Kestose (G-F-F) (G: glucose, F: fructose, hyphen between each sugar stands for glycosidic bond. Molecular weight of glucose and fructose is 180)

Product formed: 0.5 g/l for 30 min with 2 ml of enzyme, which was prepared by dissolving 0.1 mg of solid enzyme into 5 ml of water.

4. Calculate kinetic parameters(Km, Vmax) based on the following Lineweaver-      Burk plot [20점].

5. Derive the Michaelis-Menten equation [20점].