Enzyme kinetics-inhibition Problem set 2          Due May 9, 2024

1. Salicylate (aspirin) inhibits the catalytic action of glutamate dehydrogenase as presented in the following data set. Estimate the degree of inhibition.

2. Another type of inhibitor of glutamate dehydrogenase was tested and obtained the following data. Determine the type of inhibition (competitive or non-competitive) and degree of inhibition.

3.You have purified a new enzyme and want to determine its kinetic properties in the presence and absence of two inhibitors (I-1 and I-2).  The following data were obtained: Determine the type of inhibition (competitive or non-competitive) and degree of inhibition for the inhibitor I-1 and I-2.