2023-1 Enzyme Engineering     Problem set #3 (by May 31)

1.Determine inhibition constant (Ki) for the following experiments (I=0.1nmol/L).

S (nmol/L)	V (without inhibition)	V (with inhibition)
0.710	0.200	0.180
0.400	0.180	0.150
0.310	0.160	0.110
0.098	0.120	0.070
0.066	0.100	0.050
0.040	0.070	0.040

2. Determine the type of inhibition and degree of inhibition at 1 mM substrate

for the following experiments.