2022 Enzyme Engineering / Problem set #2      Due April 13

1. Determine the enzyme activity as in units/ml for the following reactions.

(1) Reaction: fructo-oligosaccharide formation from sucrose (GF→GFF) by

fructosyltransferase (liquid)

Reaction time: 1 hour, enzyme dosage: 5 ml

Product formed: 3 g/l 1-kestose (GFF). 1-Kestose has two glycosidic bonds

between two glucose and fructose molecules.

(2) Reaction: glucose isomerization (Glucose → Fructose) by glucose

isomerase (liquid)

Reaction time: 30 min, enzyme dosage: 3 ml

Product formed:  0.5 g/l fructose

2. Determine the enzyme activity as in units/mg (so-called "specific

activity") for the following reactions.

Reaction: maltose → glucose by maltase (solid)

Reaction time: 30 min, enzyme dosage: 3 ml (taken from 20 ml of

enzyme solution, where 3 mg of enzyme was dissolved)

Product formed: 0.3 g/l glucose