Enzyme Engineering Homework (Due date: April 13, 2021)

1. Determine the enzyme activity as in units/ml for the following reaction

results.

(1) Reaction: inulin (Fn, n=35) hydrolysis (Fn→nF) by endoinulinase

(liquid).  Reaction time: 30 min; enzyme dosage: 2 ml; Product

formed: 2 g/l inulobiose.

(2) Reaction: Fructooligosaccharides production from sucrose (GF → GFF:

G: glucose; F: fructose. GFF consists of 2 glycosidic bonds) by

fructosyltransferase (liquid type)

Reaction time: 60 min; enzyme dosage: 3 ml; Product formed:  3 g/l

1-kestose (GFF).

2. Determine the enzyme activity as in units/mg (so-called "specific

activity") for the following reaction results.

Reaction: glucose → gluconic acid by glucose oxidase (solid)

Reaction time: 1 hour; enzyme dosage: 2 ml (taken from 10 ml of

enzyme solution containing 2 mg of enzyme); Product formed: 3 g/l

gluconic acid.

3. Calculate amount of enzyme (liter) for the following purpose.

- Reactor working volume: 20 KL

- Reaction time: 24 hours including service time.

- Enzyme activity: 500 units/ml

- Enzyme required: 5 units/g substrate

- Substrate concentration: 20 g/L