2020 Enzyme Engineering / Problem set #2

(Submission deadline is April 23 pm 10, not accepted after the deadline)

1. Determine the enzyme activity as in units/ml for the following reaction

results.

(1) Reaction: inulin (Fn, n=35) hydrolysis (Fn→nF) by endoinulinase (liquid)

Reaction time: 1 hour, enzyme dosage: 3 ml

Product formed: 3 g/l inulobiose (F2). Inulobiose has a glycosidic bond

between two fructose molecules.

(2) Reaction: glucose isomerization (Glucose → Fructose) by glucose

isomerase (liquid)

Reaction time: 20 min, enzyme dosage: 2 ml

Product formed:  2 g/l fructose

2. Determine the enzyme activity as in units/mg (so-called "specific

activity") for the following reaction results.

Reaction: glucose → gluconic acid by glucose oxidase (solid)

Reaction time: 1 hour, enzyme dosage: 2 ml (taken from 10 ml of

enzyme solution which 2 mg of enzyme was dissolved)

Product formed: 3 g/l gluconic acid